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Volume 13 Issue 4
Mar.  2022
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Lei Chang, Mengfan Li, Shipeng Shao, Chen Li, Shanshan Ai, Boxin Xue, Yingping Hou, Yiwen Zhang, Ruifeng Li, Xiaoying Fan, Aibin He, Cheng Li, Yujie Sun. Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells[J]. Protein&Cell, 2022, 13(4): 258-280. doi: 10.1007/s13238-020-00794-8
Citation: Lei Chang, Mengfan Li, Shipeng Shao, Chen Li, Shanshan Ai, Boxin Xue, Yingping Hou, Yiwen Zhang, Ruifeng Li, Xiaoying Fan, Aibin He, Cheng Li, Yujie Sun. Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells[J]. Protein&Cell, 2022, 13(4): 258-280. doi: 10.1007/s13238-020-00794-8
shu

Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells

  • 1 State Key Laboratory of Membrane Biology, School of Life Sciences, and Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing, 100871, China;

  • 2 Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China;

  • 3 Center for Bioinformatics, School of Life Sciences, Peking University, Beijing, 100871, China;

  • 4 Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Peking University, Beijing, 100871, China;

  • 5 Center for Statistical Science, Peking University, Beijing, 100871, China;

  • 6 Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, 510530, China

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  • Received Date: June 28, 2020
  • Available Online: November 04, 2020
  • Published Date: March 23, 2022
    Graphical Abstract
    • Abstract
  • The eukaryotic genome is folded into higher-order conformation accompanied with constrained dynamics for coordinated genome functions. However, the molecular machinery underlying these hierarchically organized three-dimensional (3D) chromatin architecture and dynamics remains poorly understood. Here by combining imaging and sequencing, we studied the role of lamin B1 in chromatin architecture and dynamics. We found that lamin B1 depletion leads to detachment of lamina-associated domains (LADs) from the nuclear periphery accompanied with global chromatin redistribution and decompaction. Consequently, the inter-chromosomal as well as inter-compartment interactions are increased, but the structure of topologically associating domains (TADs) is not affected. Using live-cell genomic loci tracking, we further proved that depletion of lamin B1 leads to increased chromatin dynamics, owing to chromatin decompaction and redistribution toward nucleoplasm. Taken together, our data suggest that lamin B1 and chromatin interactions at the nuclear periphery promote LAD maintenance, chromatin compaction, genomic compartmentalization into chromosome territories and A/B compartments and confine chromatin dynamics, supporting their crucial roles in chromatin higher-order structure and chromatin dynamics.
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