Current Articles

2021, Volume 12,  Issue 12

Circular RNA (circRNA) is a novel class of single-stranded RNAs with a closed loop structure. Their expression is dynamically regulated and shows spatiotemporal patterns among cell types, tissues and developmental stages. CircRNAs have important biological functions in many physiological processes, and their aberrant expression is implicated in many human diseases. In this cover paper, the authors focus on the translational potential of using human blood circRNAs as liquid biopsy biomarkers for human diseases and highlight their abundant expression, essential biological functions and significant correlations to human diseases in various components of peripheral blood, including whole blood, blood cells and extracellular vesicles. In addition, they also summarize the current knowledge of blood circRNA biomarkers for disease diagnosis or prognosis.

Recollection
Fern botanist Ren-Chang Ching and his taxonomic system
Xunfeng Xu, Lei Fu
2021, 12(12): 903-905. doi: 10.1007/s13238-020-00758-y
Abstract:
Commentary
RNA m6A meets transposable elements and chromatin
Chenxi He, Fei Lan
2021, 12(12): 906-910. doi: 10.1007/s13238-021-00859-2
Abstract:
Review
The potential of using blood circular RNA as liquid biopsy biomarker for human diseases
Guoxia Wen, Tong Zhou, Wanjun Gu
2021, 12(12): 911-946. doi: 10.1007/s13238-020-00799-3
Abstract:
Circular RNA (circRNA) is a novel class of single-stranded RNAs with a closed loop structure. The majority of circRNAs are formed by a back-splicing process in pre-mRNA splicing. Their expression is dynamically regulated and shows spatiotemporal patterns among cell types, tissues and developmental stages. CircRNAs have important biological functions in many physiological processes, and their aberrant expression is implicated in many human diseases. Due to their high stability, circRNAs are becoming promising biomarkers in many human diseases, such as cardiovascular diseases, autoimmune diseases and human cancers. In this review, we focus on the translational potential of using human blood circRNAs as liquid biopsy biomarkers for human diseases. We highlight their abundant expression, essential biological functions and significant correlations to human diseases in various components of peripheral blood, including whole blood, blood cells and extracellular vesicles. In addition, we summarize the current knowledge of blood circRNA biomarkers for disease diagnosis or prognosis.
Research Article
Generation of developmentally competent oocytes and fertile mice from parthenogenetic embryonic stem cells
Chenglei Tian, Linlin Liu, Ming Zeng, Xiaoyan Sheng, Dai Heng, Lingling Wang, Xiaoying Ye, David L. Keefe, Lin Liu
2021, 12(12): 947-964. doi: 10.1007/s13238-021-00865-4
Abstract:
Parthenogenetic embryos, created by activation and diploidization of oocytes, arrest at mid-gestation for defective paternal imprints, which impair placental development. Also, viable offspring has not been obtained without genetic manipulation from parthenogenetic embryonic stem cells (pESCs) derived from parthenogenetic embryos, presumably attributable to their aberrant imprinting. We show that an unlimited number of oocytes can be derived from pESCs and produce healthy offspring. Moreover, normal expression of imprinted genes is found in the germ cells and the mice. pESCs exhibited imprinting consistent with exclusively maternal lineage, and higher X-chromosome activation compared to female ESCs derived from the same mouse genetic background. pESCs differentiated into primordial germ cell-like cells (PGCLCs) and formed oocytes following in vivo transplantation into kidney capsule that produced fertile pups and reconstituted ovarian endocrine function. The transcriptome and methylation of imprinted and X-linked genes in pESC-PGCLCs closely resembled those of in vivo produced PGCs, consistent with efficient reprogramming of methylation and genomic imprinting. These results demonstrate that amplification of germ cells through parthenogenesis faithfully maintains maternal imprinting, offering a promising route for deriving functional oocytes and having potential in rebuilding ovarian endocrine function.
Letter
METTL9 mediated N1-histidine methylation of zinc transporters is required for tumor growth
Mengyue Lv, Dan Cao, Liwen Zhang, Chi Hu, Shukai Li, Panrui Zhang, Lianbang Zhu, Xiao Yi, Chaoliang Li, Alin Yang, Zhentao Yang, Yi Zhu, Kaiguang Zhang, Wen Pan
2021, 12(12): 965-970. doi: 10.1007/s13238-021-00857-4
Abstract:
Correction
Correction to: The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication
Wan Zhao, Junjie Zhu, Hong Lu, Jiaming Zhu, Fei Jiang, Wei Wang, Lan Luo, Le Kang, Feng Cui
2021, 12(12): 971-975. doi: 10.1007/s13238-021-00854-7
Abstract: