Latest Articles

Correction to: Neuroendocrine characteristics of induced pluripotent stem cells from polycystic ovary syndrome women
Zheying Min, Yue Zhao, Jing Hang, Yun Ren, Tao Tan, Yong Fan, Yang Yu
 doi: 10.1007/s13238-019-00664-y
Qi Fang: Affective interweave with patients
Tanping Fu, Wenli Duan, Mingyan Chen, Shuyang Zhang
 doi: 10.1007/s13238-019-00667-9
Kuo-Hua Sun: The founder of physiologic psychology and child psychology in China
Yimeng Wang, Yanyan Qian
 doi: 10.1007/s13238-019-00666-w
Lipid droplets and mitochondria are anchored during brown adipocyte differentiation
Liujuan Cui, Mirza Ahmed Hammad, Shuyan Zhang, Bin Liang, Pingsheng Liu
 doi: 10.1007/s13238-019-00661-1
Binding to m6A RNA promotes YTHDF2-mediated phase separation
Jiahua Wang, Liyong Wang, Jianbo Diao, Yujiang Geno Shi, Yang Shi, Honghui Ma, Hongjie Shen
 doi: 10.1007/s13238-019-00660-2
Professor Haowen Xu: The founder of exercise biochemistry in China
Wei Gong, Yijing Shen, Jiaqi Bao, Yike Ying, Han Zhou, Zhifeng Wu
 doi: 10.1007/s13238-019-00663-z
Chi-Nan Hu-Pioneer of Chinese neuroscience and cognitive science
Yunjin Wang, Yanyan Qian
 doi: 10.1007/s13238-019-00658-w
Genetic evidence for asymmetric blocking of higher-order chromatin structure by CTCF/cohesin
Yujia Lu, Jia Shou, Zhilian Jia, Yonghu Wu, Jinhuan Li, Ya Guo, Qiang Wu
 doi: 10.1007/s13238-019-00656-y
Shu-szi Sin: A Chinese pioneer biologist, ancient agronomist, and educator
Qun Xie, Yan-ping Cheng, Lei Fu
 doi: 10.1007/s13238-019-00657-x
A pioneer of modern Chinese Physiology: Dr. Robert Kho-Seng Lim
Fang Zhang
 doi: 10.1007/s13238-019-00655-z
The role of telomere-binding modulators in pluripotent stem cells
Feng Li, Yuanlong Ge, Dan Liu, Zhou Songyang
 doi: 10.1007/s13238-019-0651-y
Pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs), ESCs derived by somatic cell nuclear transfer (ntESCs), and induced pluripotent stem cells (iPSCs) have unlimited capacity for self-renewal and pluripotency and can give rise to all types of somatic cells. In order to maintain their self-renewal and pluripotency, PSCs need to preserve their telomere length and homeostasis. In recent years, increasing studies have shown that telomere reprogramming is essential for stem cell pluripotency maintenance and its induced pluripotency process. Telomere-associated proteins are not only required for telomere maintenance in both stem cells, their extra-telomeric functions have also been found to be critical as well. Here, we will discuss how telomeres and telomere-associated factors participate and regulate the maintenance of stem cell pluripotency.
Continuous culture of urine-derived bladder cancer cells for precision medicine
Shuai Jiang, Jiaqi Wang, Chen Yang, Renke Tan, Jun Hou, Yuan Shi, Huihui Zhang, Shiyu Ma, Jianan Wang, Mengmeng Zhang, George Philips, Zengxia Li, Jian Ma, Wanjun Yu, Guohua Wang, Yuanming Wu, Richard Schlegel, Huina Wang, Shanbo Cao, Jianming Guo, Xuefeng Liu, Yongjun Dang
 doi: 10.1007/s13238-019-0649-5
FBXW7 E3 ubiquitin ligase: degrading, not degrading, or being degraded
Huiyin Lan, Yi Sun
 doi: 10.1007/s13238-019-0652-x
Long non-coding RNA LncKdm2b regulates cortical neuronal differentiation by cis-activating Kdm2b
Wei Li, Wenchen Shen, Bo Zhang, Kuan Tian, Yamu Li, Lili Mu, Zhiyuan Luo, Xiaoling Zhong, Xudong Wu, Ying Liu, Yan Zhou
 doi: 10.1007/s13238-019-0650-z
The mechanisms underlying spatial and temporal control of cortical neurogenesis of the brain are largely elusive. Long non-coding RNAs (lncRNAs) have emerged as essential cell fate regulators. Here we found LncKdm2b (also known as Kancr), a lncRNA divergently transcribed from a bidirectional promoter of Kdm2b, is transiently expressed during early differentiation of cortical projection neurons. Interestingly, Kdm2b's transcription is positively regulated in cis by LncKdm2b, which has intrinsic-activating function and facilitates a permissive chromatin environment at the Kdm2b's promoter by associating with hnRNPAB. Lineage tracing experiments and phenotypic analyses indicated LncKdm2b and Kdm2b are crucial in proper differentiation and migration of cortical projection neurons. These observations unveiled a lncRNA-dependent machinery in regulating cortical neuronal differentiation.
Core transcriptional signatures of phase change in the migratory locust
Pengcheng Yang, Li Hou, Xianhui Wang, Le Kang
 doi: 10.1007/s13238-019-0648-6
Phenotypic plasticity plays fundamental roles in successful adaptation of animals in response to environmental variations. Here, to reveal the transcriptome reprogramming in locust phase change, a typical phenotypic plasticity, we conducted a comprehensive analysis of multiple phase-related transcriptomic datasets of the migratory locust. We defined PhaseCore genes according to their contribution to phase differentiation by the adjustment for confounding principal components analysis algorithm (AC-PCA). Compared with other genes, PhaseCore genes predicted phase status with over 87.5% accuracy and displayed more unique gene attributes including the faster evolution rate, higher CpG content and higher specific expression level. Then, we identified 20 transcription factors (TFs) named PhaseCoreTF genes that are associated with the regulation of PhaseCore genes. Finally, we experimentally verified the regulatory roles of three representative TFs (Hr4, Hr46, and grh) in phase change by RNAi. Our findings revealed that core transcriptional signatures are involved in the global regulation of locust phase changes, suggesting a potential common mechanism underlying phenotypic plasticity in insects. The expression and network data are accessible in an online resource called LocustMine (
Tissue-resident memory-like ILCs: innate counterparts of TRM cells
Xianwei Wang, Zhigang Tian, Hui Peng
 doi: 10.1007/s13238-019-0647-7
Innate lymphoid cells (ILCs) are defined as lymphocytes that lack RAG recombinase and do not express diverse antigen receptors; however, recent studies have revealed the adaptive features of ILCs. Mouse cytomegalovirus (MCMV)-and cytokine-induced memory natural killer (NK) cells circulate in the blood and are referred to as conventional memory NK cells. In contrast, virusand hapten-induced memory NK cells, hapten-induced memory ILC1s, and cytokine-induced memory-like ILC2s exhibit long-term residency in the liver or lung, and are referred to as tissue-resident memory ILCs. Considering their similar migration patterns and memory potential, tissue-resident memory ILCs could be regarded as innate counterparts of resident memory T (TRM) cells. Both tissue-resident memory ILCs and TRM cells share common characteristics in terms of dynamics, phenotype, and molecular regulation. The emergence of ILC memory expands the basic biology of ILCs and prompts us to re-examine their functions in disease progression. This review discusses the evidence supporting tissue-resident memory NK cells and other memory ILC subsets, compares them with TRM cells, and highlights key unsolved questions in this emerging field.
Ubiquitin is double-phosphorylated by PINK1 for enhanced pH-sensitivity of conformational switch
Shang-Xiang Ye, Zhou Gong, Ju Yang, Yu-Xin An, Zhu Liu, Qun Zhao, Ewen Lescop, Xu Dong, Chun Tang
 doi: 10.1007/s13238-019-0644-x
New paradigms on hematopoietic stem cell differentiation
Hui Cheng, Zhaofeng Zheng, Tao Cheng
 doi: 10.1007/s13238-019-0633-0
Ever since hematopoietic stem cells (HSCs) were first identified half a century ago,their differentiation roadmap has been extensively studied.The classical model of hematopoiesis has long held as a dogma that HSCs reside at the top of a hierarchy in which HSCs possess self-renewal capacity and can progressively give rise to all blood lineage cells.However,over the past several years,with advances in single cell technologies,this developmental scheme has been challenged.In this review,we discuss the evidence supporting heterogeneity within HSC and progenitor populations as well as the hierarchical models revised by novel approaches mainly in mouse system.These evolving views provide further understanding of hematopoiesis and highlight the complexity of hematopoietic differentiation.
Modeling neuropsychiatric disorders using human induced pluripotent stem cells
Meiyan Wang, Lei Zhang, Fred H. Gage
 doi: 10.1007/s13238-019-0638-8
Neuropsychiatric disorders are complex disorders characterized by heterogeneous genetic variations, variable symptoms, and widespread changes in anatomical pathology. In the context of neuropsychiatric disorders, limited access to relevant tissue types presents challenges for understanding disease etiology and developing effective treatments. Induced pluripotent stem cells (iPSCs) reprogrammed from patient somatic cells offer an opportunity to recapitulate disease development in relevant cell types, and they provide novel approaches for understanding disease mechanisms and for development of effective treatments. Here we review recent progress and challenges in differentiation paradigms for generating disease-relevant cells and recent studies of neuropsychiatric disorders using human pluripotent stem cell (hPSC) models where cellular phenotypes linked to disease have been reported. The use of iPSC-based disease models holds great promise for understanding disease mechanisms and supporting discovery of effective treatments.
Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/ Cas9-mediated gene correction
Si Wang, Zheying Min, Qianzhao Ji, Lingling Geng, Yao Su, Zunpeng Liu, Huifang Hu, Lixia Wang, Weiqi Zhang, Keiichiro Suzuiki, Yu Huang, Puyao Zhang, Tie-Shan Tang, Jing Qu, Yang Yu, Guang-Hui Liu, Jie Qiao
 doi: 10.1007/s13238-019-0623-2
Cockayne syndrome (CS) is a rare autosomal recessive inherited disorder characterized by a variety of clinical features, including increased sensitivity to sunlight, progressive neurological abnormalities, and the appearance of premature aging. However, the pathogenesis of CS remains unclear due to the limitations of current disease models. Here, we generate integration-free induced pluripotent stem cells (iPSCs) from fibroblasts from a CS patient bearing mutations in CSB/ERCC6 gene and further derive isogenic genecorrected CS-iPSCs (GC-iPSCs) using the CRISPR/Cas9 system. CS-associated phenotypic defects are recapitulated in CS-iPSC-derived mesenchymal stem cells (MSCs) and neural stem cells (NSCs), both of which display increased susceptibility to DNA damage stress. Premature aging defects in CS-MSCs are rescued by the targeted correction of mutant ERCC6. We next map the transcriptomic landscapes in CS-iPSCs and GC-iPSCs and their somatic stem cell derivatives (MSCs and NSCs) in the absence or presence of ultraviolet (UV) and replicative stresses, revealing that defects in DNA repair account for CS pathologies. Moreover, we generate autologous GC-MSCs free of pathogenic mutation under a cGMP (Current Good Manufacturing Practice)-compliant condition, which hold potential for use as improved biomaterials for future stem cell replacement therapy for CS. Collectively, our models demonstrate novel disease features and molecular mechanisms and lay a foundation for the development of novel therapeutic strategies to treat CS.
Current advances in haploid stem cells
Tongtong Cui, Zhikun Li, Qi Zhou, Wei Li
 doi: 10.1007/s13238-019-0625-0
Diploidy is the typical genomic mode in all mammals. Haploid stem cells are artificial cell lines experimentally derived in vitro in the form of different types of stem cells, which combine the characteristics of haploidy with a broad developmental potential and open the possibility to uncover biological mysteries at a genomic scale. To date, a multitude of haploid stem cell types from mouse, rat, monkey and humans have been derived, as more are in development. They have been applied in high-throughput genetic screens and mammalian assisted reproduction. Here, we review the generation, unique properties and broad applications of these remarkable cells.
Professor Cuifen Huang: a great molecular geneticist and the founder of genetic engineering in China
Wei Gong, Leyi Cui, Yike Ying, Yijing Shen, Jiaqi Bao
 doi: 10.1007/s13238-019-0620-5
Richang Cao: pioneer advocate of dialectical materialism applied to psychological research
Baoyuan Zhang
 doi: 10.1007/s13238-019-0615-2
Correction to: Developing potent PROTACs tools for selective degradation of HDAC6 protein
Zixuan An, Wenxing Lv, Shang Su, Wei Wu, Yu Rao
 doi: 10.1007/s13238-019-0613-4
Tonghua Liu: A life dedicated to clinical pathology
Lin Dong, Tanping Fu, Junyi Pang, Zhiyong Liang, Wenli Duan
 doi: 10.1007/s13238-018-0601-0
Professor Li-Pin. King: a famous physiologist in China
Wei Gong, Fangfang Wang, Yike Ying
 doi: 10.1007/s13238-018-0582-z
Zing-Yang Kuo and behavior epigenesis based on animal experiments
Yanyan Qian, Wei Chen, Benyu Guo
 doi: 10.1007/s13238-018-0516-9
A 110-year-old wise man: Professor Libin T. Cheng, one of the founders of biochemistry and nutrition in China
He Zhang
 doi: 10.1007/s13238-017-0428-0
Retraction Note to: DDB1 and Cul4 are necessary for gene silencing and heterochromatin formation at pericentromeric regions in Neurospora
Yingqiong Cao, Jicheng Wei, Silu Yang, Jinquan Sun, Hui Xu, Ying Wang, Yuanbiao Zhao, Qun He
 doi: 10.1007/s13238-014-0079-3
RETRACTED ARTICLE: DDB1 and Cul4 are necessary for gene silencing and heterochromatin formation at pericentromeric regions in Neurospora
Yingqiong Cao, Jicheng Wei, Silu Yang, Jinquan Sun, Hui Xu, Ying Wang, Yuanbiao Zhao, Qun He
 doi: 10.1007/s13238-014-0067-7
Current issue
  • ISSN1674-800X
  • EISSN1674-8018
  • IF (2018) 7.575

Microbiota and Human Health

May 2018
Volume 9
Issue 5
pp: 395-510

Metabolism and Disease

May 2018
Volume 9
Issue 2
pp: 141-237

Therapeutic Antibodies

May 2018
Volume 9
Issue 1
pp: 1-139


Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang Beijing 100101, China

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